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  colon carcinoma HCT116

Overall Design109 single-cell human transcriptomes were analyzed in total; 96 using nanoliter volume sample processing on a microfluidic platform, Nextera library prep (biological replicates); 3 using the SMARTer cDNA synthesis kit, Nextera library prep (biological replicates); 3 using the Transplex cDNA synthesis kit, Nextera library prep (biological replicates); 7 using the Ovation Nugen cDNA synthesis kit (biological replicates) where 3 used Nextera library prep and 4 used NEBNext library prep. In addition, 4 bulk RNA samples were sequenced: bulk RNA generated using ~1 million pooled cells was used to make bulk libraries, 2 of which were made using SMARTer cDNA synthesis kit (technical replicates) and 2 made using Superscript RT kit with no amplification (technical replicates). All 4 bulk samples were made into libraries using Nextera.
SummaryWe generated single-cell transcriptomes from a large number of single cells using several commercially available platforms, in both microliter and nanoliter volumes, and compared performance between them. We benchmarked each method to conventional RNA-seq of the same sample using bulk total RNA, as well as to multiplexed qPCR, which is the current gold standard for quantitative single-cell gene expression analysis. In doing so, we were able to systematically evaluate the sensitivity, precision, and accuracy of various approaches to single-cell RNA-seq. Our results show that it is possible to use single-cell RNA-seq to perform quantitative transcriptome measurements of individual cells, that it is possible to obtain quantitative and accurate gene expression measurements with a relatively small number of sequencing reads, and that when such measurements are performed on large numbers of cells, one can recapitulate the bulk transcriptome complexity, and the distributions of gene expression levels found by single-cell qPCR.
Dataset viewGSE51254
PMID24141493

Samples in colon carcinoma HCT116

Displaying 61-70 of 109 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE51254GSM1241201Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 48colon carcinoma HCT116
GSE51254GSM1241202Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 49colon carcinoma HCT116
GSE51254GSM1241203Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 50colon carcinoma HCT116
GSE51254GSM1241204Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 51colon carcinoma HCT116
GSE51254GSM1241205Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 52colon carcinoma HCT116
GSE51254GSM1241206Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 53colon carcinoma HCT116
GSE51254GSM1241207Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 54colon carcinoma HCT116
GSE51254GSM1241208Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 55colon carcinoma HCT116
GSE51254GSM1241209Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 56colon carcinoma HCT116
GSE51254GSM1241210Illumina HiSeq 2000Homo sapiensSingle Cell C1 Microfluidics 57colon carcinoma HCT116

Gene rank in colon carcinoma HCT116

Displaying 20281-20290 of 20378 results.
Rank orderGene SymbolEnsembl ID
20281FMO2ENSG00000094963
20282ADAMTS20ENSG00000173157
20283PLCZ1ENSG00000139151
20284LOC100128176
20285LOC286177
20286LOC284950
20287ASB5ENSG00000164122
20288SLC35G6ENSG00000259224
20289SGK1ENSG00000118515
20290BTLAENSG00000186265