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  d4 BTAG+ cells

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in d4 BTAG+ cells

Displaying 1-5 of 5 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643177AB 5500xl Genetic AnalyzerHomo sapiensd4_hetd4 BTAG+ cells
GSE67259GSM1643178AB 5500xl Genetic AnalyzerHomo sapiensd4_ko1d4 BTAG+ cells
GSE67259GSM1643179AB 5500xl Genetic AnalyzerHomo sapiensd4_ko2d4 BTAG+ cells
GSE67259GSM1643149AB 5500xl Genetic AnalyzerHomo sapiensd4BTAG+#1d4 BTAG+ cells
GSE67259GSM1643150AB 5500xl Genetic AnalyzerHomo sapiensd4BTAG+#2d4 BTAG+ cells

Gene rank in d4 BTAG+ cells

Displaying 21-30 of 20748 results.
Rank orderGene SymbolEnsembl ID
21HSP90AA1ENSG00000080824
22RPL3ENSG00000100316
23RPL27AENSG00000166441
24FTH1ENSG00000167996
25GAPDHENSG00000111640
26PRDX1ENSG00000117450
27RPL13ENSG00000167526
28EEF1GENSG00000254772
29RPL31ENSG00000071082
30RPL24ENSG00000114391