scRNASeqDB - CellView Site

Overall Design	6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.
Summary	Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell.
Dataset view	GSE65364
PMID	26902283

Displaying 1-8 of 8 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE65364	GSM2039755	HiSeq X Ten	Homo sapiens	scTrio_HepG2_1_RNA	HepG2 cell line
GSE65364	GSM2039757	HiSeq X Ten	Homo sapiens	scTrio_HepG2_2_RNA	HepG2 cell line
GSE65364	GSM2039759	HiSeq X Ten	Homo sapiens	scTrio_HepG2_3_RNA	HepG2 cell line
GSE65364	GSM2039761	HiSeq X Ten	Homo sapiens	scTrio_HepG2_4_RNA	HepG2 cell line
GSE65364	GSM2039763	HiSeq X Ten	Homo sapiens	scTrio_HepG2_5_RNA	HepG2 cell line
GSE65364	GSM2039765	HiSeq X Ten	Homo sapiens	scTrio_HepG2_6_RNA	HepG2 cell line
GSE65364	GSM2039769	HiSeq X Ten	Homo sapiens	scRNA-seq_HepG2_1	HepG2 cell line
GSE65364	GSM2039770	HiSeq X Ten	Homo sapiens	scRNA-seq_HepG2_2	HepG2 cell line

Displaying 11-20 of 15622 results.