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  hiPSC

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in hiPSC

Displaying 1-10 of 11 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643143AB 5500xl Genetic AnalyzerHomo sapienshiPSC#1hiPSC
GSE67259GSM1643144AB 5500xl Genetic AnalyzerHomo sapienshiPSC#2hiPSC
GSE67259GSM1643155AB 5500xl Genetic AnalyzerHomo sapienshiPSC*1hiPSC
GSE67259GSM1643156AB 5500xl Genetic AnalyzerHomo sapienshiPSC*2hiPSC
GSE67259GSM1643159AB 5500xl Genetic AnalyzerHomo sapiens585A1_hiPSC#1hiPSC
GSE67259GSM1643160AB 5500xl Genetic AnalyzerHomo sapiens585A1_hiPSC#2hiPSC
GSE67259GSM1643165AB 5500xl Genetic AnalyzerHomo sapiensiPSC_wt1hiPSC
GSE67259GSM1643166AB 5500xl Genetic AnalyzerHomo sapiensiPSC_wt2hiPSC
GSE67259GSM1643167AB 5500xl Genetic AnalyzerHomo sapiensiPSC_hethiPSC
GSE67259GSM1643168AB 5500xl Genetic AnalyzerHomo sapiensiPSC_ko1hiPSC

Gene rank in hiPSC

Displaying 41-50 of 20748 results.
Rank orderGene SymbolEnsembl ID
41RPS15AENSG00000134419
42TUBBENSG00000183311
43RPL37AENSG00000197756
44LOC100652733
45RPL17ENSG00000265681
46RPS13ENSG00000110700
47RPL37ENSG00000145592
48RPS25ENSG00000118181
49TUBA1BENSG00000123416
50RPS4XENSG00000198034