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  Primordial Germ Cells[male 7 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 7 week gestation]

Displaying 1-10 of 40 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677479Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc1Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677480Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc2Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677481Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc3Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677482Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc4Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677483Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc5Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677484Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc6Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677485Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc7Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677486Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc8Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677487Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc9Primordial Germ Cells[male 7 week gestation]
GSE63818GSM1677488Illumina HiSeq 2500Homo sapiensM_PGC_7W_embryo1_sc10Primordial Germ Cells[male 7 week gestation]

Gene rank in Primordial Germ Cells[male 7 week gestation]

Displaying 21281-21290 of 21483 results.
Rank orderGene SymbolEnsembl ID
21281UPK2ENSG00000110375
21282UPK3AENSG00000100373
21283UPP2ENSG00000007001
21284UROC1ENSG00000159650
21285USF1ENSG00000158773
21286USH1CENSG00000006611
21287USH1GENSG00000182040
21288USHBP1ENSG00000130307
21289USP12-AS2ENSG00000230641
21290USP17L10ENSG00000231396