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  glioblastoma [Pro+Cla]

Overall DesignOperative specimens from five glioblastoma patients (MGH26, MGH28, MGH29, MGH30, MGH31) were acutely dissociated, depleted for CD45+ inflammatory cells and then sorted as single cells (576 samples). Population controls for each tumor were isolated by sorting 2000-10000 cells and processed in parallel (5 population control samples). Single cells from two established cell lines, GBM6 and GBM8, were also sorted as single cells (192 samples).
SummaryWe report transcriptomes from 430 single glioblastoma cells isolated from 5 individual tumors and 102 single cells from gliomasphere cells lines generated using SMART-seq. In addition, we report population RNA-seq from the five tumors as well as RNA-seq from cell lines derived from 3 tumors (MGH26, MGH28, MGH31) cultured under serum free (GSC) and differentiated (DGC) conditions. This dataset highlights intratumoral heterogeneity with regards to the expression of de novo derived transcriptional modules and established subtype classifiers.
Dataset viewGSE57872
PMID24925914

Samples in glioblastoma [Pro+Cla]

Displaying 1-10 of 20 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE57872GSM1395508Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_B02glioblastoma [Pro+Cla]
GSE57872GSM1395511Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_B05glioblastoma [Pro+Cla]
GSE57872GSM1395516Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_B10glioblastoma [Pro+Cla]
GSE57872GSM1395531Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_D01glioblastoma [Pro+Cla]
GSE57872GSM1395807Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_C01glioblastoma [Pro+Cla]
GSE57872GSM1395564Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_F10glioblastoma [Pro+Cla]
GSE57872GSM1395825Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_D07glioblastoma [Pro+Cla]
GSE57872GSM1395835Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_E05glioblastoma [Pro+Cla]
GSE57872GSM1395581Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH26-2_H03glioblastoma [Pro+Cla]
GSE57872GSM1395854Illumina HiSeq 2500Homo sapiensSingle cell mRNA-seq_MGH30_F12glioblastoma [Pro+Cla]

Gene rank in glioblastoma [Pro+Cla]

Displaying 141-150 of 5942 results.
Rank orderGene SymbolEnsembl ID
141TBC1D8BENSG00000133138
142CNN3ENSG00000117519
143HNRNPRENSG00000125944
144EIF3IENSG00000084623
145RPL23AENSG00000198242
146NDUFA4ENSG00000189043
147MAGED2ENSG00000102316
148MIR4721
149NRCAMENSG00000091129
150ATP1A2ENSG00000018625