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  cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR

Overall Design26 Llive and 19 Fixed cultured hESCs were prepared and sequenced using both FRISCR and TritonX-100 Lysis as proof of principal for FRSCR.
SummaryThe human neocortex is created from diverse progenitors that are intermixed with multiple cell types in the prenatal germinal zones. These progenitors have been difficult to profile with unbiased transcriptomics since progenitors-particularly radial glia (RG)-are rare cell types, defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed a method called FRSCR for transcriptome profiling of individual fixed, stained, and sorted cells. After validation of FRSCR with human embryonic stem cells, we profiled primary human RG that constitute only 1% of the mid-gestation cortex. These data showed that RG could be classified into ventricle zone-enriched RG (vRG) that expressed ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that expressed HOPX. Our study identified the first markers and molecular profiles of vRG and oRG cells, and provides an essential step for understanding molecular networks that control the development and lineage of human neocortical progenitors. Furthermore, FRSCR allows targeted single-cell transcriptomic profiling of many tissues that currently lack live-cell markers.
Dataset viewGSE71858
PMID26524239

Samples in cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR

Displaying 1-10 of 12 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE71858GSM1846957Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-18]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846958Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-19]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846960Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-20]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846961Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-21]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846962Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-22]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846963Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457350-23]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846976Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457351-14]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846977Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457351-15]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846978Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457351-16]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR
GSE71858GSM1846979Illumina HiSeq 2500Homo sapiensFixed_PLRC [US-1457351-17]cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR

Gene rank in cultured embryonic stem cells (H1) DAPI+ Fixed cells FRSCR

Displaying 131-140 of 17315 results.
Rank orderGene SymbolEnsembl ID
131RPL12ENSG00000197958
132NCRNA00188
133NGFRAP1
134NDUFS5ENSG00000168653
135RPS9ENSG00000170889
136POU5F1ENSG00000204531
137SRP9ENSG00000143742
138NME2ENSG00000243678
139RPL34ENSG00000109475
140YWHAEENSG00000108953