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  d8 BTAG+ cells

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in d8 BTAG+ cells

Displaying 1-2 of 2 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643153AB 5500xl Genetic AnalyzerHomo sapiensd8BTAG+#1d8 BTAG+ cells
GSE67259GSM1643154AB 5500xl Genetic AnalyzerHomo sapiensd8BTAG+#2d8 BTAG+ cells

Gene rank in d8 BTAG+ cells

Displaying 20701-20710 of 20748 results.
Rank orderGene SymbolEnsembl ID
20701LOC158696
20702F9ENSG00000101981
20703SOX3ENSG00000134595
20704CDR1ENSG00000184258
20705CXorf18
20706SPANXDENSG00000196406
20707FMR1-AS1ENSG00000268066
20708FMR1NBENSG00000176988
20709MAGEA9BENSG00000267978
20710HSFX1ENSG00000171116