scRNASeqDB - CellView Site

Overall Design	Single GM12878 cells were picked and RNA-seq libraries were generated using the SMART-seq protocol. We also carried out RNA-seq experiments on pools of 10, 30 and 100 cells, on 100pg and 10ng of total RNA, and on pools of 10 cells that were subsequently split into 10 separate sample and processed as if they were single cells in order to assess technical variation in our experiments.
Summary	RNA-seq transcriptome measurements are typically performed by isolating RNA from large numbers of cells in culture or tissues. While highly informative, such experiments mask the variability in gene expression patterns that exists between individual cells. To gain insight into the dynamics of gene expression on the level of single-cells, we have carried out the transcriptomes of single-cells from the GM12878 cell line using RNA-seq.
Dataset view	GSE44618
PMID	24299736

Displaying 1-10 of 28 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE44618	GSM1087862	Illumina HiSeq 2000	Homo sapiens	12522-100mers-GM12878_183-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087863	Illumina HiSeq 2000	Homo sapiens	12523-100mers-GM12878_184-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087864	Illumina HiSeq 2000	Homo sapiens	12524-100mers-GM12878_185-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087865	Illumina HiSeq 2000	Homo sapiens	12534-100mers-GM12878_186-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087866	Illumina HiSeq 2000	Homo sapiens	12535-100mers-GM12878_187-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087867	Illumina HiSeq 2000	Homo sapiens	12536-100mers-GM12878_188-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087868	Illumina HiSeq 2000	Homo sapiens	12537-100mers-GM12878_189-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087869	Illumina HiSeq 2000	Homo sapiens	12538-100mers-GM12878_190-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087870	Illumina HiSeq 2000	Homo sapiens	12539-100mers-GM12878_191-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087871	Illumina HiSeq 2000	Homo sapiens	12540-100mers-GM12878_192-TopHat-1.4.1-GENCODE-V13	GM12878

Displaying 1-10 of 26201 results.