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  Primordial Germ Cells[male 10 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 10 week gestation]

Displaying 1-10 of 19 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677519Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc2Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677520Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc3Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677521Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc4Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677522Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc5Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677523Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc6Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677524Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc7Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677525Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc8Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677526Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc9Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677527Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc10Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677528Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc11Primordial Germ Cells[male 10 week gestation]

Gene rank in Primordial Germ Cells[male 10 week gestation]

Displaying 1-10 of 21483 results.
Rank orderGene SymbolEnsembl ID
1EEF1A1ENSG00000156508
2RPS6ENSG00000137154
3RPL4ENSG00000174444
4RPLP0ENSG00000089157
5RPL7AENSG00000148303
6RPSAENSG00000168028
7GNB2L1
8EEF1GENSG00000254772
9RPS7ENSG00000171863
10RPS3AENSG00000145425