scRNASeqDB - CellView Site

Overall Design	To achieve a deep RNA sequencing profile of CTCs at the single cell level, we applied a novel inertial focusing-enhanced device, the CTC-iChip, which allows high efficiency negative depletion of normal blood cells, leaving unattached CTCs in solution where they can be selected and analyzed as single cells (Pubmed ID 23552373). CTCs were then subjected to single cell RNA-sequencing (Pubmed ID 20203668).
Summary	Circulating Tumor Cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. To determine the relevance of ECM protein expression to human disease, CTCs were isolated from the blood of metastatic PDAC patients and subjected to single cell RNA-sequencing. Analysis of 7 pancreatic CTCs from 3 patients revealed that the majority expressed keratins defining their epithelial origin. A total of 13 of 60 extracellular protein genes enriched in mouse CTCs (see GEO GSE51372) were expressed at high levels (>100 rpm) in at least one human pancreatic CTC. Human SPARC was the only gene found at high levels in all human pancreatic CTCs.
Dataset view	GSE60407
PMID	25242334

Displaying 1-7 of 7 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE60407	GSM1478319	AB 5500xl Genetic Analyzer	Homo sapiens	HP1_2	circulating pancreas tumor cell
GSE60407	GSM1478320	AB 5500xl Genetic Analyzer	Homo sapiens	HP1_3	circulating pancreas tumor cell
GSE60407	GSM1478321	AB 5500xl Genetic Analyzer	Homo sapiens	HP2_2	circulating pancreas tumor cell
GSE60407	GSM1478322	AB 5500xl Genetic Analyzer	Homo sapiens	HP2_3	circulating pancreas tumor cell
GSE60407	GSM1478323	AB 5500xl Genetic Analyzer	Homo sapiens	HP2_4	circulating pancreas tumor cell
GSE60407	GSM1478324	AB 5500xl Genetic Analyzer	Homo sapiens	HP2_5	circulating pancreas tumor cell
GSE60407	GSM1478325	AB 5500xl Genetic Analyzer	Homo sapiens	HP3_1	circulating pancreas tumor cell

Displaying 1-10 of 11561 results.