scRNASeqDB - CellView Site

Overall Design	6 single human HepG2 cell line cells were sequenced using the newly developed scTrio-seq, other 2 HepG2 cells were sequenced using scRNA-seq and other 2 HepG2 cells were sequenced using scRRBS as technique control. 6 single mouse embryonic stem cells (mESCs) were sequenced using the newly developted scTrio-seq. Meanwhile, two scRNA-seq and two scRRBS were also completed using two mESCs separately. 26 single cells from hepatocellular carcinoma were sequenced using scTrio-seq to analyze the regulation relations between three omics of cancer cells.
Summary	Single cell genome, DNA methylome, and transcriptome sequencing has been achieved separately. However, to analyze the regulation of RNA expression by genetic and epigenetic factors within an individual cell, it is necessary to analyze these omics simultaneously from the same single cell. Here we developed a single cell triple omics sequencing technique- scTrio-seq, to analyze the genome, DNA methylome, and transcriptome concurrently of a mammalian cell.
Dataset view	GSE65364
PMID	26902283

Displaying 1-10 of 26 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE65364	GSM1593768	Illumina HiSeq 2000	Homo sapiens	Ca_01_RNA	liver cancer cell
GSE65364	GSM1593770	Illumina HiSeq 2000	Homo sapiens	Ca_02_RNA	liver cancer cell
GSE65364	GSM1593772	Illumina HiSeq 2000	Homo sapiens	Ca_03_RNA	liver cancer cell
GSE65364	GSM1593774	Illumina HiSeq 2000	Homo sapiens	Ca_04_RNA	liver cancer cell
GSE65364	GSM1593776	Illumina HiSeq 2000	Homo sapiens	Ca_05_RNA	liver cancer cell
GSE65364	GSM1593778	Illumina HiSeq 2000	Homo sapiens	Ca_06_RNA	liver cancer cell
GSE65364	GSM1593780	Illumina HiSeq 2000	Homo sapiens	Ca_07_RNA	liver cancer cell
GSE65364	GSM1593782	Illumina HiSeq 2000	Homo sapiens	Ca_08_RNA	liver cancer cell
GSE65364	GSM1593784	Illumina HiSeq 2000	Homo sapiens	Ca_09_RNA	liver cancer cell
GSE65364	GSM1593786	Illumina HiSeq 2000	Homo sapiens	Ca_10_RNA	liver cancer cell

Displaying 1-10 of 15622 results.