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Dataset View [GSE71318]

SeriesGSE71318
TitleTracing the expression of circular RNAs in human preimplantation embryos
Year2016
CountryChina
ArticleNot set
PMIDNA
Bio ProjectBioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA291062
SraSRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP061636
Overall DesginWe sequenced individual single cells or embryos at seven consecutive stages (mature oocytes, zygotes, 2-cell, 4-cell, 8-cell embryos, morulae, and blastocysts) and single hESC using single-cell RNA seq.
SummaryWe systematically analyzed transcriptome in individual human oocytes and preimplantation embryos with a new single-cell RNA seq method we recently developed.
Experimental ProtocolThe RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina); RNA-seq (single-cell)
Data processingIllumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene with Refseq ID.
Illumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene in Refseq.
PlatformGPL16791
Public OnPublic on May 13 2016

Cell Groups

Differential Expression Gene List

KEGG GO Others   

Gene SymbolEnsembl IDFDR
AAMPENSG000001278370.00244361821776968
DNMT1ENSG000001308160.00244361821776968
SNHG6ENSG000002459100.00244361821776968
SYNE1ENSG000001310180.00244361821776968
KPNA7ENSG000001854670.00305111622114271
WDR7ENSG000000911570.00440366931226456
AGKENSG000000065300.00467642070654502
AKAP13ENSG000001707760.00467642070654502
BOD1ENSG000001459190.00467642070654502
CFLARENSG000000034020.00467642070654502
Displaying 1-10 of 575 results.