Dataset View [GSE71318]

SeriesGSE71318
TitleTracing the expression of circular RNAs in human preimplantation embryos
Year2016
CountryChina
ArticleNot set
PMIDNA
Bio ProjectBioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA291062
SraSRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP061636
Overall DesginWe sequenced individual single cells or embryos at seven consecutive stages (mature oocytes, zygotes, 2-cell, 4-cell, 8-cell embryos, morulae, and blastocysts) and single hESC using single-cell RNA seq.
SummaryWe systematically analyzed transcriptome in individual human oocytes and preimplantation embryos with a new single-cell RNA seq method we recently developed.
Experimental ProtocolThe RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina); RNA-seq (single-cell)
Data processingIllumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene with Refseq ID.
Illumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene in Refseq.
PlatformGPL16791
Public OnPublic on May 13 2016

Cell Groups

Created with Highcharts 4.2.5CH1CH2Cell Clusterhuman 2 cellhuman 4 cellhuman 8 cellhuman early blastocsythuman ICMhuman late blastoycsthuman middle blastocysthuman MII oocytehuman morulahuman stem cellshuman TEhuman zygote-150-125-100-75-50-250255075100125150175200-150-125-100-75-50-250255075100125

Differential Expression Gene List

KEGG GO Others   

Gene SymbolEnsembl IDFDR
AAMPENSG000001278370.00244361821776968
DNMT1ENSG000001308160.00244361821776968
SNHG6ENSG000002459100.00244361821776968
SYNE1ENSG000001310180.00244361821776968
KPNA7ENSG000001854670.00305111622114271
WDR7ENSG000000911570.00440366931226456
AGKENSG000000065300.00467642070654502
AKAP13ENSG000001707760.00467642070654502
BOD1ENSG000001459190.00467642070654502
CFLARENSG000000034020.00467642070654502
Displaying 1-10 of 575 results.