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Dataset View [GSE71318]

Series	GSE71318
Title	Tracing the expression of circular RNAs in human preimplantation embryos
Year	2016
Country	China
Article	Not set
PMID	NA
Bio Project	BioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA291062
Sra	SRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP061636
Overall Desgin	We sequenced individual single cells or embryos at seven consecutive stages (mature oocytes, zygotes, 2-cell, 4-cell, 8-cell embryos, morulae, and blastocysts) and single hESC using single-cell RNA seq.
Summary	We systematically analyzed transcriptome in individual human oocytes and preimplantation embryos with a new single-cell RNA seq method we recently developed.
Experimental Protocol	The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina) The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina); RNA-seq (single-cell)
Data processing	Illumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene with Refseq ID. Illumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene in Refseq.
Platform	GPL16791
Public On	Public on May 13 2016

Cell Groups

Differential Expression Gene List

human 2 cell_VS_human 4 cell human 2 cell_VS_human 8 cell human 2 cell_VS_human early blastocsyt human 2 cell_VS_human ICM human 2 cell_VS_human late blastoycst human 2 cell_VS_human middle blastocyst human 2 cell_VS_human MII oocyte human 2 cell_VS_human morula human 2 cell_VS_human stem cells human 2 cell_VS_human TE human 2 cell_VS_human zygote human 4 cell_VS_human 8 cell human 4 cell_VS_human early blastocsyt human 4 cell_VS_human ICM human 4 cell_VS_human late blastoycst human 4 cell_VS_human middle blastocyst human 4 cell_VS_human MII oocyte human 4 cell_VS_human morula human 4 cell_VS_human stem cells human 4 cell_VS_human TE human 4 cell_VS_human zygote human 8 cell_VS_human early blastocsyt human 8 cell_VS_human ICM human 8 cell_VS_human late blastoycst human 8 cell_VS_human middle blastocyst human 8 cell_VS_human MII oocyte human 8 cell_VS_human morula human 8 cell_VS_human stem cells human 8 cell_VS_human TE human 8 cell_VS_human zygote human early blastocsyt_VS_human ICM human early blastocsyt_VS_human late blastoycst human early blastocsyt_VS_human middle blastocyst human early blastocsyt_VS_human MII oocyte human early blastocsyt_VS_human morula human early blastocsyt_VS_human stem cells human early blastocsyt_VS_human TE human early blastocsyt_VS_human zygote human ICM_VS_human late blastoycst human ICM_VS_human middle blastocyst human ICM_VS_human MII oocyte human ICM_VS_human morula human ICM_VS_human stem cells human ICM_VS_human TE human ICM_VS_human zygote human late blastoycst_VS_human middle blastocyst human late blastoycst_VS_human MII oocyte human late blastoycst_VS_human morula human late blastoycst_VS_human stem cells human late blastoycst_VS_human TE human late blastoycst_VS_human zygote human middle blastocyst_VS_human MII oocyte human middle blastocyst_VS_human morula human middle blastocyst_VS_human stem cells human middle blastocyst_VS_human TE human middle blastocyst_VS_human zygote human MII oocyte_VS_human morula human MII oocyte_VS_human stem cells human MII oocyte_VS_human TE human MII oocyte_VS_human zygote human morula_VS_human stem cells human morula_VS_human TE human morula_VS_human zygote human stem cells_VS_human TE human stem cells_VS_human zygote human TE_VS_human zygote

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