Loading

Dataset View [GSE70580]

SeriesGSE70580
TitleSingle cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)
Year2016
CountrySweden
ArticleMjösberg J,Sandberg R,Friberg D,Theorell J,Konya V,Picelli S,Forkel M,Björklund ÅK.The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing.Nature immunology.2016 Apr
PMID26878113
Bio ProjectBioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA289086
SraSRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP060416
Overall DesginSequencing libraries were prepared from FACS sorted individual ILCs with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)
SummarySingle cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs) from three independent tonsil donors.
Experimental ProtocolcDNA was generated from FACS sorted cells using to Smart-seq2 (Picelli et al. Nature Methods 2013); Nextera XT DNA Sample Preparation Index Kit (24 index primers, Illumina)
Data processingAll reads were aligned to the human (hg19) genomes using STAR (Dobin et al. 2013); Non-uniquely mapped reads were removed; Rpkmforgenes (available at http://sandberg.cmb.ki.se/rnaseq/), options -readcount -fulltranscript -mRNAnorm -rmnameoverlap -u, was used to generate RPKM values and read counts. We used a file with unique positions from Storvall et al. 2013 PLOS ONE. Gene annotations used were transcripts in RefSeq and Ensembl (Feb 2013) and ERCC-spike in annotations.; Genome_build: hg19; Supplementary_files_format_and_content: gene expression values (tab-delimited text)
PlatformGPL11154
Public OnPublic on Jan 27 2016

Cell Groups

Differential Expression Gene List

KEGG GO Others   

Gene SymbolEnsembl IDFDR
HS2ST1ENSG000001539360.00884395471529742
CTC-498J12.30.008873820761265
CENPPENSG000001883120.00890393903426339
VASH1ENSG000000712460.00890633567998996
PPP1R1CENSG000001507220.00890812359646549
KLRG1ENSG000001391870.00892409984781138
C15orf52ENSG000001885490.00896493865812438
SLC16A10ENSG000001123940.00899386256958512
SUV39H1ENSG000001019450.0090366363551642
CRKENSG000001671930.00904678415308316
Displaying 1021-1030 of 1063 results.