Loading

Dataset View [GSE70580]

SeriesGSE70580
TitleSingle cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)
Year2016
CountrySweden
ArticleMjösberg J,Sandberg R,Friberg D,Theorell J,Konya V,Picelli S,Forkel M,Björklund ÅK.The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing.Nature immunology.2016 Apr
PMID26878113
Bio ProjectBioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA289086
SraSRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP060416
Overall DesginSequencing libraries were prepared from FACS sorted individual ILCs with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)
SummarySingle cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs) from three independent tonsil donors.
Experimental ProtocolcDNA was generated from FACS sorted cells using to Smart-seq2 (Picelli et al. Nature Methods 2013); Nextera XT DNA Sample Preparation Index Kit (24 index primers, Illumina)
Data processingAll reads were aligned to the human (hg19) genomes using STAR (Dobin et al. 2013); Non-uniquely mapped reads were removed; Rpkmforgenes (available at http://sandberg.cmb.ki.se/rnaseq/), options -readcount -fulltranscript -mRNAnorm -rmnameoverlap -u, was used to generate RPKM values and read counts. We used a file with unique positions from Storvall et al. 2013 PLOS ONE. Gene annotations used were transcripts in RefSeq and Ensembl (Feb 2013) and ERCC-spike in annotations.; Genome_build: hg19; Supplementary_files_format_and_content: gene expression values (tab-delimited text)
PlatformGPL11154
Public OnPublic on Jan 27 2016

Cell Groups

Differential Expression Gene List

KEGG GO Others   

Gene SymbolEnsembl IDFDR
GPCPD1ENSG000001257721.17780092690188e-17
XCL2ENSG000001431852.00636760172217e-17
TNFSF8ENSG000001069523.08620221943697e-17
KDSRENSG000001195376.1138602898493e-17
PXNENSG000000891597.3851198046261e-17
MTRNR2L2ENSG000002710438.82317364146551e-17
FGRENSG000000009381.08371798458753e-16
MTRNR2L8ENSG000002558231.10208710979772e-16
YDJCENSG000001611791.51747540143825e-16
THEMISENSG000001726732.26269664915553e-16
Displaying 21-30 of 1343 results.