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Dataset View [GSE71318]

SeriesGSE71318
TitleTracing the expression of circular RNAs in human preimplantation embryos
Year2016
CountryChina
ArticleNot set
PMIDNA
Bio ProjectBioProject: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA291062
SraSRA: http://www.ncbi.nlm.nih.gov/sra?term=SRP061636
Overall DesginWe sequenced individual single cells or embryos at seven consecutive stages (mature oocytes, zygotes, 2-cell, 4-cell, 8-cell embryos, morulae, and blastocysts) and single hESC using single-cell RNA seq.
SummaryWe systematically analyzed transcriptome in individual human oocytes and preimplantation embryos with a new single-cell RNA seq method we recently developed.
Experimental ProtocolThe RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina)
The RNAs in individual oocyte or embryo were reverse transcribed and amplified using the single RNA-seq method we recently developed.; Libraries were constructed with TruSeq DNA library preparation kit (Illumina); RNA-seq (single-cell)
Data processingIllumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene with Refseq ID.
Illumina CASAVA version 1.8 were used to the basecalling.; Reads were trimmed to remove the adapter sequences and low quality bases.; RNA-seq reads were aligned to the human refference genome (hg19) using Tophat software; Raw read counts and RPKM calculated by HTSeq; Genome_build: hg19; Supplementary_files_format_and_content: txt files reflect RPKM of each gene in Refseq.
PlatformGPL16791
Public OnPublic on May 13 2016

Cell Groups

Differential Expression Gene List

KEGG GO Others   

Gene SymbolEnsembl IDFDR
ARID4AENSG000000322190.00528716395134372
GAPDHENSG000001116400.00528716395134372
KMT2EENSG000000054830.00528716395134372
PAIP1ENSG000001722390.00528716395134372
RPSAENSG000001680280.00528716395134372
RSRC2ENSG000001110110.00528716395134372
RUVBL2ENSG000001832070.00528716395134372
ZNF638ENSG000000752920.00528716395134372
ZNHIT3ENSG000002736110.00528716395134372
FBXO11ENSG000001380810.00606512509926498
Displaying 1-10 of 91 results.