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  2-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in 2-cell blastomere

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160120Illumina HiSeq 2000Homo sapienshuman 2-cell 12-cell blastomere
GSE44183GSM1160121Illumina HiSeq 2000Homo sapienshuman 2-cell 52-cell blastomere
GSE44183GSM1160122Illumina HiSeq 2000Homo sapienshuman 2-cell 62-cell blastomere

Gene rank in 2-cell blastomere

Displaying 14721-14730 of 14758 results.
Rank orderGene SymbolEnsembl ID
14721TEX11ENSG00000120498
14722TEX13AENSG00000268629
14723TFDP3ENSG00000183434
14724TMEM31ENSG00000179363
14725TMEM47ENSG00000147027
14726TMSB15AENSG00000158164
14727TMSB15BENSG00000158427
14728TROENSG00000067445
14729TSPAN6ENSG00000000003
14730UBL4AENSG00000102178