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  2-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in 2-cell blastomere

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160120Illumina HiSeq 2000Homo sapienshuman 2-cell 12-cell blastomere
GSE44183GSM1160121Illumina HiSeq 2000Homo sapienshuman 2-cell 52-cell blastomere
GSE44183GSM1160122Illumina HiSeq 2000Homo sapienshuman 2-cell 62-cell blastomere

Gene rank in 2-cell blastomere

Displaying 14601-14610 of 14758 results.
Rank orderGene SymbolEnsembl ID
14601DDX26B
14602DGKKENSG00000274588
14603DUSP21ENSG00000189037
14604DUSP9ENSG00000130829
14605DYNLT3ENSG00000165169
14606EGFL6ENSG00000198759
14607ELF4ENSG00000102034
14608FAM127CENSG00000212747
14609FAM133AENSG00000179083
14610FAM9AENSG00000183304