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  4-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in 4-cell blastomere

Displaying 1-4 of 4 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160126Illumina HiSeq 2000Homo sapienshuman 4-cell 44-cell blastomere
GSE44183GSM1160125Illumina HiSeq 2000Homo sapienshuman 4-cell 34-cell blastomere
GSE44183GSM1160124Illumina HiSeq 2000Homo sapienshuman 4-cell 24-cell blastomere
GSE44183GSM1160123Illumina HiSeq 2000Homo sapienshuman 4-cell 14-cell blastomere

Gene rank in 4-cell blastomere

Displaying 14711-14720 of 14758 results.
Rank orderGene SymbolEnsembl ID
14711SATL1ENSG00000184788
14712SH3BGRLENSG00000131171
14713SLC7A3ENSG00000165349
14714SMPXENSG00000091482
14715SPANXCENSG00000198573
14716SPANXDENSG00000196406
14717SPANXE
14718SPANXN5ENSG00000204363
14719SPIN2BENSG00000186787
14720SPIN4ENSG00000186767