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  4-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183
PMID23892778

Samples in 4-cell blastomere

Displaying 1-4 of 4 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160123Illumina HiSeq 2000Homo sapienshuman 4-cell 14-cell blastomere
GSE44183GSM1160124Illumina HiSeq 2000Homo sapienshuman 4-cell 24-cell blastomere
GSE44183GSM1160125Illumina HiSeq 2000Homo sapienshuman 4-cell 34-cell blastomere
GSE44183GSM1160126Illumina HiSeq 2000Homo sapienshuman 4-cell 44-cell blastomere

Gene rank in 4-cell blastomere

Displaying 71-80 of 14758 results.
Rank orderGene SymbolEnsembl ID
71RNF34ENSG00000170633
72DNAJA1ENSG00000086061
73WFDC2ENSG00000101443
74AURKAENSG00000087586
75RPL12ENSG00000197958
76RPL18AENSG00000105640
77CDK2AP2ENSG00000167797
78RPL18ENSG00000063177
79RANENSG00000132341
80CCNIENSG00000118816