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  8-cell blastomere

Overall Designsingle-cell RNA-seq of human and mouse blastomeres
SummaryMammalian preimplantation development is a complex process involving dramatic changes in the transcriptional architecture. Through single-cell RNA-sequencing (RNA-seq), we report here a comprehensive analysis of transcriptome dynamics from oocyte to morula in both human and mouse embryos. Based on single nucleotide variants (SNVs) in blastomere mRNAs and paternal-specific SNPs, we identify novel stage-specific monoallelic expression patterns for a significant portion of polymorphic gene transcripts (25-53%). By weighted gene co-expression network analysis (WGCNA), we find that each developmental stage can be concisely delineated by a small number of functional modules of co-expressed genes. This result indicates a sequential order of transcriptional changes in pathways of cell cycle, gene regulation, translation, and metabolism in a step-wise fashion from cleavage to morula. Cross-species comparisons reveal that the majority of human stage-specific modules (7 out of 9) are remarkably preserved, only to diverge in developmental specificity and timing in mice. We further identify conserved key members (or hub genes) of the human and mouse networks. These genes represent novel candidates that are likely key players in driving mammalian preimplantation development. Collectively, we demonstrate that mammalian preimplantation development is orchestrated by evolutionarily conserved genetic programs that diverge in developmental timing. Our results provide a valuable resource to dissect gene regulatory mechanism underlying progressive development of early mammalian embryos.
Dataset viewGSE44183

Samples in 8-cell blastomere

Displaying 1-10 of 10 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE44183GSM1160127Illumina HiSeq 2000Homo sapienshuman 8-cell 3a8-cell blastomere
GSE44183GSM1160128Illumina HiSeq 2000Homo sapienshuman 8-cell 6a8-cell blastomere
GSE44183GSM1160129Illumina HiSeq 2000Homo sapienshuman 8-cell 7a8-cell blastomere
GSE44183GSM1160130Illumina HiSeq 2000Homo sapienshuman 8-cell 1b8-cell blastomere
GSE44183GSM1160131Illumina HiSeq 2000Homo sapienshuman 8-cell 2b8-cell blastomere
GSE44183GSM1160132Illumina HiSeq 2000Homo sapienshuman 8-cell 3b8-cell blastomere
GSE44183GSM1160133Illumina HiSeq 2000Homo sapienshuman 8-cell 4b8-cell blastomere
GSE44183GSM1160134Illumina HiSeq 2000Homo sapienshuman 8-cell 5b8-cell blastomere
GSE44183GSM1160135Illumina HiSeq 2000Homo sapienshuman 8-cell 3c8-cell blastomere
GSE44183GSM1160136Illumina HiSeq 2000Homo sapienshuman 8-cell 5c8-cell blastomere

Gene rank in 8-cell blastomere