scRNASeqDB - CellView Site

Overall Design	Single GM12878 cells were picked and RNA-seq libraries were generated using the SMART-seq protocol. We also carried out RNA-seq experiments on pools of 10, 30 and 100 cells, on 100pg and 10ng of total RNA, and on pools of 10 cells that were subsequently split into 10 separate sample and processed as if they were single cells in order to assess technical variation in our experiments.
Summary	RNA-seq transcriptome measurements are typically performed by isolating RNA from large numbers of cells in culture or tissues. While highly informative, such experiments mask the variability in gene expression patterns that exists between individual cells. To gain insight into the dynamics of gene expression on the level of single-cells, we have carried out the transcriptomes of single-cells from the GM12878 cell line using RNA-seq.
Dataset view	GSE44618
PMID	24299736

Displaying 21-28 of 28 results.

Series	Sample	Instrument	Organism	Title	Cell Source
GSE44618	GSM1087886	Illumina HiSeq 2000	Homo sapiens	13284-100mers-GM12878-237-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087887	Illumina HiSeq 2000	Homo sapiens	13285-100mers-GM12878-238-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087888	Illumina HiSeq 2000	Homo sapiens	13286-100mers-GM12878-239-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087889	Illumina HiSeq 2000	Homo sapiens	13287-100mers-GM12878-240-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087890	Illumina HiSeq 2000	Homo sapiens	13288-100mers-GM12878-242-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087891	Illumina HiSeq 2000	Homo sapiens	13289-100mers-GM12878-243-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087892	Illumina HiSeq 2000	Homo sapiens	13290-100mers-GM12878-244-TopHat-1.4.1-GENCODE-V13	GM12878
GSE44618	GSM1087893	Illumina HiSeq 2000	Homo sapiens	13291-100mers-GM12878-245-TopHat-1.4.1-GENCODE-V13	GM12878

Displaying 11-20 of 26201 results.