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  HEK293T

Overall DesignSequencing libraries from cDNA was generated using in-house produced or commercial Tn5 and in a variety of tagmentation buffers and conditions.
SummaryMassively parallel DNA sequencing of thousands of samples in a single machine-run is now possible, but the preparation of the individual sequencing libraries is expensive and time-consuming. Tagmentation-based library construction, using the Tn5 transposase, is efficient for generating sequencing libraries but currently relies on undisclosed reagents, which severely limits development of novel applications and the execution of large scale projects. Here, we present simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library construction. We further show how molecular crowding agents both modulate library lengths and enable efficient tagmentation from sub-picogram amounts of cDNA. Comparison of single-cell RNA-sequencing libraries generated using produced and commercial Tn5 demonstrated equal performances in terms of gene detection and library characteristics. Finally, as naked Tn5 can be annealed to any oligonucleotide of choice, for example molecular barcodes in single-cell assays or methylated oligonucleotides for bisulfite sequencing, custom Tn5 production and tagmentation enables innovation in sequencing-based applications.
Dataset viewGSE58652
PMID25079858

Samples in HEK293T

Displaying 41-42 of 42 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE58652GSM1416450Illumina HiSeq 2000Homo sapiensXT_500pg_rep2HEK293T
GSE58652GSM1416451Illumina HiSeq 2000Homo sapiensXT_500pg_rep3HEK293T

Gene rank in HEK293T

Displaying 41-50 of 19442 results.
Rank orderGene SymbolEnsembl ID
41HSPA1BENSG00000204388
42ATP5BENSG00000110955
43FAUENSG00000149806
44RPL30ENSG00000156482
45HSP90AB1ENSG00000096384
46RPS6ENSG00000137154
47AGPAT1ENSG00000204310
48RANENSG00000132341
49BRD2ENSG00000204256
50RPL4ENSG00000174444