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  Human Skeletal Muscle Myoblasts (HSMM)

Overall DesignWe selected primary human myoblasts as a model system of cell differentiation to investigate whether ordering cells by progress revealed new regulators of the process. We sequenced RNA-Seq libraries from each of several hundred cells taken over a time-course of serum-induced differentiation.
SummarySingle-cell expression profiling by RNA-Seq promises to exploit cell-to-cell variation in gene expression to reveal regulatory circuitry governing cell differentiation and other biological processes. Here, we describe Monocle, a novel unsupervised algorithm for ordering cells by progress through differentiation that dramatically increases temporal resolution of expression measurements. This reordering unmasks switch-like changes in expression of key regulatory factors, reveals sequentially organized waves of gene regulation, and exposes regulators of cell differentiation. A functional screen confirms that a number of these regulators dramatically alter the efficiency of myoblast differentiation, demonstrating that single-cell expression analysis with Monocle can uncover new regulators even in well-studied systems.
Dataset viewGSE52529

Samples in Human Skeletal Muscle Myoblasts (HSMM)

Displaying 21-30 of 372 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE52529GSM1269141Illumina HiSeq 2500Homo sapiensCell T24_CT_H02Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269142Illumina HiSeq 2500Homo sapiensCell T24_CT_H03Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269143Illumina HiSeq 2500Homo sapiensCell T24_CT_H04Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269144Illumina HiSeq 2500Homo sapiensCell T24_CT_H05Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269145Illumina HiSeq 2500Homo sapiensCell T24_CT_H06Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269146Illumina HiSeq 2500Homo sapiensCell T24_CT_H07Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269147Illumina HiSeq 2500Homo sapiensCell T24_CT_H08Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269148Illumina HiSeq 2500Homo sapiensCell T24_CT_H09Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269149Illumina HiSeq 2500Homo sapiensCell T24_CT_H10Human Skeletal Muscle Myoblasts (HSMM)
GSE52529GSM1269150Illumina HiSeq 2500Homo sapiensCell T24_CT_H11Human Skeletal Muscle Myoblasts (HSMM)

Gene rank in Human Skeletal Muscle Myoblasts (HSMM)