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  PDX primary renal cell carcinoma

Overall DesignIn order to identify successful clonal propagation from patient to PDX samples and understand pathogenesis from primary to metastatic RCC, we performed whole-exome sequencing (WES, n=4) and matched aCGH (n=4) on bulk tumor samples. And we utilized single-cell RNA sequencing (scRNA-seq) to model and dissect functional heterogeneity acroass primary and metastatic RCC tumors. We checked whether of capturing live one cell, not more cells, in microfluidics by fluorescent microscopic observation. To construct RNA sequencing libraries, we performed further quality controls including adequate quantities and qualities of amplified transcriptomes respectively from single cells. Tumor cells from the parental mRCC (n=34), PDX-mRCC (n=36) and PDX-pRCC (n=46) were finally analyzed in this study after filtering out poor quality cells.
SummaryClear cell renal cell carcinoma (ccRCC) initiated from the renal epithelium is the most prevalent histological type of adult kidney cancers. Dissecting intratumoral heterogeneity (ITH) of ccRCC has leveraged to extend our knowledge on how primary tumors harboring driver mutations evolve and spread to other sites. The cellular fractions within and across the primary (pRCC) and metastatic RCC (mRCC) are heterogeneous in both their genetic and biological features determining the variability in clinical aggressiveness and sensitivity to the therapy. To achieve sustainable therapeutic benefit with targeted agents in mRCC, the effective target should focus on signaling pathways that are related to driver mutations occurred early in the clonal evolution of the disease and thus should be common to primary tumor and metastatic sites. Considering that extensive genetic heterogeneity may result in drug response variability among patients and treatment resistance, the tailored strategies for metastatic RCC is urgently needed. Here, we analyze single-cell RNA-seq (scRNA-seq) data from a matched primary RCC (pRCC) and lung metastasis (mRCC) to dissect ITH at the highest resolution to date with the objective of discovering the better therapeutic regimen.
Dataset viewGSE73121

Samples in PDX primary renal cell carcinoma

Displaying 21-30 of 47 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE73121GSM1887273Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_53PDX primary renal cell carcinoma
GSE73121GSM1887274Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_55PDX primary renal cell carcinoma
GSE73121GSM1887275Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_56PDX primary renal cell carcinoma
GSE73121GSM1887276Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_57PDX primary renal cell carcinoma
GSE73121GSM1887277Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_58PDX primary renal cell carcinoma
GSE73121GSM1887278Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_59PDX primary renal cell carcinoma
GSE73121GSM1887279Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_60PDX primary renal cell carcinoma
GSE73121GSM1887280Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_61PDX primary renal cell carcinoma
GSE73121GSM1887281Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_62PDX primary renal cell carcinoma
GSE73121GSM1887282Illumina HiSeq 2500Homo sapiensPDX_pRCC_SC_65PDX primary renal cell carcinoma

Gene rank in PDX primary renal cell carcinoma

Displaying 71-80 of 18370 results.
Rank orderGene SymbolEnsembl ID