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  Primitive Endoderm

Overall DesignSingle-Cell RNA-seq
SummaryHere we provide fundamental insights into early human development by single-cell RNA-sequencing of human and mouse preimplantation embryos. We elucidate conserved transcriptional programs along with those that are human-specific. Importantly, we validate our RNA-sequencing findings at the protein level, which further reveals differences in human and mouse embryo gene expression. For example, we identify several genes exclusively expressed in the human pluripotent epiblast including the transcription factor KLF17. Key components of the TGF-β signaling pathway including NODAL, GDF3, TGFBR1/ALK5, LEFTY1, SMAD2, SMAD4 and TDGF1 are also enriched in the human epiblast. Intriguingly, inhibition of TGF-β signaling abrogates NANOG expression in human epiblast cells, consistent with a requirement for this pathway in pluripotency. Although key trophectoderm factors Id2, Elf5, and Eomes are exclusively localized to this lineage in the mouse, the human orthologues are either absent or expressed in alternative lineages. Importantly, we also identify genes with conserved expression dynamics including Foxa2/FOXA2, which we show is restricted to the primitive endoderm in both human and mouse embryos. Comparisons of the human epiblast to existing embryonic stem cells (hESCs) reveals conservation of pluripotency but also additional pathways more enriched in hESCs. Our analysis highlights significant differences in human preimplantation development compared to mouse and provides a molecular blueprint to understand human embryogenesis and its relationship to stem cells.
Dataset viewGSE66507

Samples in Primitive Endoderm

Displaying 1-6 of 6 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE66507GSM1624232Illumina HiSeq 2000Homo sapiensPE8.1Primitive Endoderm
GSE66507GSM1868810Illumina HiSeq 2000Homo sapiensPE4.1Primitive Endoderm
GSE66507GSM1868811Illumina HiSeq 2000Homo sapiensPE5.1Primitive Endoderm
GSE66507GSM1868812Illumina HiSeq 2000Homo sapiensPE5.2Primitive Endoderm
GSE66507GSM1868813Illumina HiSeq 2000Homo sapiensPE5.3Primitive Endoderm
GSE66507GSM1868814Illumina HiSeq 2000Homo sapiensPE5.4Primitive Endoderm

Gene rank in Primitive Endoderm

Displaying 1-10 of 21732 results.
Rank orderGene SymbolEnsembl ID