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  Primordial Germ Cells[female 17 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[female 17 week gestation]

Displaying 1-10 of 31 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677675Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc1Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677676Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc2Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677677Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc3Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677678Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc4Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677679Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc5Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677680Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc6Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677681Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc7Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677682Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc8Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677683Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc9Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677684Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc10Primordial Germ Cells[female 17 week gestation]

Gene rank in Primordial Germ Cells[female 17 week gestation]

Displaying 21431-21440 of 21483 results.
Rank orderGene SymbolEnsembl ID
21431WWTR1-AS1ENSG00000241313
21432XAF1ENSG00000132530
21433XAGE5ENSG00000171405
21434XCL1ENSG00000143184
21435XCL2ENSG00000143185
21436XCR1ENSG00000173578
21437XGENSG00000124343
21438XIRP2ENSG00000163092
21439XKR4ENSG00000206579
21440XKR5ENSG00000275591