Loading
scRNASeqDB
Home » Search by Cell » Cell View

Cell View

Cell Information

  Primordial Germ Cells[female 17 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[female 17 week gestation]

Displaying 21-30 of 31 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677695Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc21Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677696Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc22Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677697Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc23Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677698Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc24Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677699Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc25Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677700Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc26Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677701Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc27Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677702Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc28Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677703Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc29Primordial Germ Cells[female 17 week gestation]
GSE63818GSM1677704Illumina HiSeq 2500Homo sapiensF_PGC_17W_embryo1_sc30Primordial Germ Cells[female 17 week gestation]

Gene rank in Primordial Germ Cells[female 17 week gestation]

Displaying 21461-21470 of 21483 results.
Rank orderGene SymbolEnsembl ID
21461ZNF221ENSG00000159905
21462ZNF321PENSG00000213801
21463ZNF366ENSG00000178175
21464ZNF385DENSG00000151789
21465ZNF469ENSG00000225614
21466ZNF474ENSG00000164185
21467ZNF491ENSG00000177599
21468ZNF536ENSG00000198597
21469ZNF579ENSG00000218891
21470ZNF630-AS1ENSG00000277541