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  Primordial Germ Cells[female 8 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[female 8 week gestation]

Displaying 1-10 of 27 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677647Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_sc14Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677648Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_sc15Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677649Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_sc16Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677650Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_sc17Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677651Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_sc18Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677792Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_ps1Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677793Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_ps2Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677794Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_ps3Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677795Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_ps4Primordial Germ Cells[female 8 week gestation]
GSE63818GSM1677796Illumina HiSeq 2500Homo sapiensF_PGC_8W_embryo1_ps5Primordial Germ Cells[female 8 week gestation]

Gene rank in Primordial Germ Cells[female 8 week gestation]

Displaying 21451-21460 of 21483 results.
Rank orderGene SymbolEnsembl ID
21451ZNF521ENSG00000198795
21452ZNF536ENSG00000198597
21453ZNF541ENSG00000118156
21454ZNF556ENSG00000172000
21455ZNF579ENSG00000218891
21456ZNF629ENSG00000102870
21457ZNF630-AS1ENSG00000277541
21458ZNF660ENSG00000144792
21459ZNF663PENSG00000215452
21460ZNF683ENSG00000176083