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  Primordial Germ Cells[male 10 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 10 week gestation]

Displaying 11-19 of 19 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677527Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc10Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677526Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc9Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677525Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc8Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677524Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc7Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677523Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc6Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677522Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc5Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677521Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc4Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677520Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc3Primordial Germ Cells[male 10 week gestation]
GSE63818GSM1677519Illumina HiSeq 2500Homo sapiensM_PGC_10W_embryo1_sc2Primordial Germ Cells[male 10 week gestation]

Gene rank in Primordial Germ Cells[male 10 week gestation]

Displaying 31-40 of 21483 results.
Rank orderGene SymbolEnsembl ID
31RPL12ENSG00000197958
32RPL26ENSG00000161970
33RPS13ENSG00000110700
34RPLP2ENSG00000177600
35TUBBENSG00000183311
36HSP90AA1ENSG00000080824
37RPS24ENSG00000138326
38POU5F1ENSG00000204531
39HSPA8ENSG00000109971
40RPL37AENSG00000197756