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  Primordial Germ Cells[male 11 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 11 week gestation]

Displaying 1-10 of 27 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677564Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc15Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677563Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc14Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677562Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc13Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677561Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc12Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677560Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc11Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677559Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc10Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677558Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc9Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677557Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc8Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677556Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc7Primordial Germ Cells[male 11 week gestation]
GSE63818GSM1677555Illumina HiSeq 2500Homo sapiensM_PGC_11W_embryo2_sc6Primordial Germ Cells[male 11 week gestation]

Gene rank in Primordial Germ Cells[male 11 week gestation]

Displaying 21401-21410 of 21483 results.
Rank orderGene SymbolEnsembl ID
21401ZBTB47ENSG00000114853
21402ZBTB6ENSG00000186130
21403ZBTB7BENSG00000160685
21404ZBTB7CENSG00000184828
21405ZC2HC1BENSG00000118491
21406ZC3H12BENSG00000102053
21407ZCCHC13ENSG00000187969
21408ZCCHC24ENSG00000165424
21409ZCCHC5ENSG00000179300
21410ZDHHC14ENSG00000175048