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  Primordial Germ Cells[male 19 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 19 week gestation]

Displaying 21-30 of 57 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677585Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc21Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677586Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc22Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677587Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc23Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677588Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc24Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677589Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc25Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677590Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc26Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677591Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc27Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677592Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc28Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677593Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc29Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677594Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo1_sc30Primordial Germ Cells[male 19 week gestation]

Gene rank in Primordial Germ Cells[male 19 week gestation]

Displaying 1-10 of 21483 results.
Rank orderGene SymbolEnsembl ID
1RPLP0ENSG00000089157
2RPS6ENSG00000137154
3RPSAENSG00000168028
4EEF1A1ENSG00000156508
5RPL7AENSG00000148303
6RPS7ENSG00000171863
7RPS12ENSG00000112306
8RPL5ENSG00000122406
9GNB2L1
10RPL4ENSG00000174444