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  Primordial Germ Cells[male 19 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Primordial Germ Cells[male 19 week gestation]

Displaying 51-57 of 57 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677598Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc3Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677599Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc4Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677600Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc5Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677601Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc6Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677602Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc7Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677603Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc8Primordial Germ Cells[male 19 week gestation]
GSE63818GSM1677604Illumina HiSeq 2500Homo sapiensM_PGC_19W_embryo2_sc9Primordial Germ Cells[male 19 week gestation]

Gene rank in Primordial Germ Cells[male 19 week gestation]

Displaying 21461-21470 of 21483 results.
Rank orderGene SymbolEnsembl ID
21461ZCCHC13ENSG00000187969
21462ZCCHC18ENSG00000166707
21463ZEB2-AS1ENSG00000238057
21464ZIM3ENSG00000141946
21465ZMIZ1-AS1ENSG00000224596
21466ZMYND15ENSG00000141497
21467ZNF205-AS1
21468ZNF224ENSG00000267680
21469ZNF252P-AS1ENSG00000255559
21470ZNF32-AS2ENSG00000230565