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  Somatic Cells[female 10 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Somatic Cells[female 10 week gestation]

Displaying 1-3 of 3 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677770Illumina HiSeq 2500Homo sapiensF_Soma_10W_embryo2_sc1Somatic Cells[female 10 week gestation]
GSE63818GSM1677771Illumina HiSeq 2500Homo sapiensF_Soma_10W_embryo2_sc2Somatic Cells[female 10 week gestation]
GSE63818GSM1677772Illumina HiSeq 2500Homo sapiensF_Soma_10W_embryo2_sc3Somatic Cells[female 10 week gestation]

Gene rank in Somatic Cells[female 10 week gestation]

Displaying 21421-21430 of 21483 results.
Rank orderGene SymbolEnsembl ID
21421ZNF879ENSG00000234284
21422ZNF880ENSG00000221923
21423ZNF883ENSG00000228623
21424ZNF890PENSG00000159904
21425ZNF891ENSG00000214029
21426ZNF91ENSG00000167232
21427ZNF92ENSG00000146757
21428ZNF98ENSG00000197360
21429ZNF99ENSG00000213973
21430ZNFX1ENSG00000124201