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  Somatic Cells[female 4 week gestation]

Overall DesignIn total, we analyzed the transcriptomes of 233 individual male and female human PGCs from 15 embryos at between 4 and 19 weeks of gestation as well as 86 neighboring somatic cells from 13 of these embryos using a single-cell RNA-Seq method that we developed. Furthermore, we analyzed the DNA methylomes of both male and female human gonadal PGCs as well as the neighboring somatic cells of eleven of these embryos at between 7 and 19 weeks of gestation using whole-genome bisulfite sequencing (WGBS). In total, we generated 1.3 billion 100 bp paired-end reads for transcriptome analyses and 4.1 billion 100 bp or 150 bp paired-end reads for DNA methylome analyses.
SummaryGerm cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Dataset viewGSE63818
PMID26046443

Samples in Somatic Cells[female 4 week gestation]

Displaying 1-6 of 6 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE63818GSM1677754Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc1Somatic Cells[female 4 week gestation]
GSE63818GSM1677755Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc2Somatic Cells[female 4 week gestation]
GSE63818GSM1677756Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc3Somatic Cells[female 4 week gestation]
GSE63818GSM1677757Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc4Somatic Cells[female 4 week gestation]
GSE63818GSM1677758Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc5Somatic Cells[female 4 week gestation]
GSE63818GSM1677759Illumina HiSeq 2500Homo sapiensF_Soma_4W_embryo2_sc6Somatic Cells[female 4 week gestation]

Gene rank in Somatic Cells[female 4 week gestation]

Displaying 21411-21420 of 21483 results.
Rank orderGene SymbolEnsembl ID
21411ZNF630-AS1ENSG00000277541
21412ZNF646ENSG00000167395
21413ZNF653ENSG00000161914
21414ZNF658BENSG00000198416
21415ZNF660ENSG00000144792
21416ZNF665ENSG00000197497
21417ZNF674-AS1ENSG00000230844
21418ZNF677ENSG00000197928
21419ZNF679ENSG00000197123
21420ZNF680ENSG00000173041