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  U87 human glioma cells

Overall DesignA microfluidic device that pairs sequence-barcoded mRNA capture beads with individual cells was used to barcode cDNA from individual cells which was then pre-amplified by in vitro transcription in a pool and converted into an Illumina RNA-Seq library. Libraries were generated from ~600 individual cells in parallel and extensive analysis was done on 396 cells from the U87 and MCF10a cell lines and from ~500 individual cells with extensive analysis on 247 cells from the U87 and WI-38 cell lines. Sequencing was done on the 3'-end of the transcript molecules. The first read contains cell-identifying barcodes that were present on the capture bead and the second read contains a unique molecular identifier (UMI) barcode, a lane-identifying barcode, and then the sequence of the transcript.
SummarySingle cell transcriptomics has emerged as a powerful approach to dissecting phenotypic heterogeneity in complex, unsynchronized cellular populations. However, many important biological questions demand quantitative analysis of large numbers of individual cells. Hence, new tools are urgently needed for efficient, inexpensive, and parallel manipulation of RNA from individual cells. We report a simple microfluidic platform for trapping single cell lysates in sealed, picoliter microwells capable of “printing” RNA on glass or capturing RNA on polymer beads. To demonstrate the utility of our system for single cell transcriptomics, we developed a highly scalable technology for genome-wide, single cell RNA-Seq. The current implementation of our device is pipette-operated, profiles hundreds of individual cells in parallel with library preparation costs of ~$0.10-$0.20/cell, and includes five lanes for simultaneous experiments. We anticipate that this system will ultimately serve as a general platform for large-scale single cell transcriptomics, compatible with both imaging and sequencing readouts.type = Expression profiling by high throughput sequencing
Dataset viewGSE66357
PMID26047807

Samples in U87 human glioma cells

Displaying 91-100 of 138 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE66357GSM1683235Illumina NextSeq 500Homo sapiensPS041-2_R2_0_387U87 human glioma cells
GSE66357GSM1683236Illumina NextSeq 500Homo sapiensPS041-2_R2_0_397U87 human glioma cells
GSE66357GSM1683237Illumina NextSeq 500Homo sapiensPS041-2_R2_0_405U87 human glioma cells
GSE66357GSM1683238Illumina NextSeq 500Homo sapiensPS041-2_R2_0_409U87 human glioma cells
GSE66357GSM1683239Illumina NextSeq 500Homo sapiensPS041-2_R2_0_437U87 human glioma cells
GSE66357GSM1683240Illumina NextSeq 500Homo sapiensPS041-2_R2_0_443U87 human glioma cells
GSE66357GSM1683241Illumina NextSeq 500Homo sapiensPS041-2_R2_0_448U87 human glioma cells
GSE66357GSM1683242Illumina NextSeq 500Homo sapiensPS041-2_R2_0_460U87 human glioma cells
GSE66357GSM1683243Illumina NextSeq 500Homo sapiensPS041-2_R2_0_473U87 human glioma cells
GSE66357GSM1683244Illumina NextSeq 500Homo sapiensPS041-2_R2_0_483U87 human glioma cells

Gene rank in U87 human glioma cells

Displaying 31-40 of 18975 results.
Rank orderGene SymbolEnsembl ID
31RPS2ENSG00000140988
32RPS14ENSG00000164587
33LGALS1ENSG00000100097
34RPS16ENSG00000105193
35RPS27AENSG00000143947
36RPLP1ENSG00000137818
37RPS3AENSG00000145425
38J01415.23
39RPL7ENSG00000147604
40RPL11ENSG00000142676