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  WI-38 human lung fibroblast cells

Overall DesignA microfluidic device that pairs sequence-barcoded mRNA capture beads with individual cells was used to barcode cDNA from individual cells which was then pre-amplified by in vitro transcription in a pool and converted into an Illumina RNA-Seq library. Libraries were generated from ~600 individual cells in parallel and extensive analysis was done on 396 cells from the U87 and MCF10a cell lines and from ~500 individual cells with extensive analysis on 247 cells from the U87 and WI-38 cell lines. Sequencing was done on the 3'-end of the transcript molecules. The first read contains cell-identifying barcodes that were present on the capture bead and the second read contains a unique molecular identifier (UMI) barcode, a lane-identifying barcode, and then the sequence of the transcript.
SummarySingle cell transcriptomics has emerged as a powerful approach to dissecting phenotypic heterogeneity in complex, unsynchronized cellular populations. However, many important biological questions demand quantitative analysis of large numbers of individual cells. Hence, new tools are urgently needed for efficient, inexpensive, and parallel manipulation of RNA from individual cells. We report a simple microfluidic platform for trapping single cell lysates in sealed, picoliter microwells capable of “printing” RNA on glass or capturing RNA on polymer beads. To demonstrate the utility of our system for single cell transcriptomics, we developed a highly scalable technology for genome-wide, single cell RNA-Seq. The current implementation of our device is pipette-operated, profiles hundreds of individual cells in parallel with library preparation costs of ~$0.10-$0.20/cell, and includes five lanes for simultaneous experiments. We anticipate that this system will ultimately serve as a general platform for large-scale single cell transcriptomics, compatible with both imaging and sequencing readouts.type = Expression profiling by high throughput sequencing
Dataset viewGSE66357
PMID26047807

Samples in WI-38 human lung fibroblast cells

Displaying 31-40 of 45 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE66357GSM1683313Illumina NextSeq 500Homo sapiensPS041-2_R2_1_67WI-38 human lung fibroblast cells
GSE66357GSM1683314Illumina NextSeq 500Homo sapiensPS041-2_R2_1_725WI-38 human lung fibroblast cells
GSE66357GSM1683315Illumina NextSeq 500Homo sapiensPS041-2_R2_1_726WI-38 human lung fibroblast cells
GSE66357GSM1683316Illumina NextSeq 500Homo sapiensPS041-2_R2_1_762WI-38 human lung fibroblast cells
GSE66357GSM1683317Illumina NextSeq 500Homo sapiensPS041-2_R2_1_77WI-38 human lung fibroblast cells
GSE66357GSM1683318Illumina NextSeq 500Homo sapiensPS041-2_R2_1_786WI-38 human lung fibroblast cells
GSE66357GSM1683319Illumina NextSeq 500Homo sapiensPS041-2_R2_1_787WI-38 human lung fibroblast cells
GSE66357GSM1683320Illumina NextSeq 500Homo sapiensPS041-2_R2_1_805WI-38 human lung fibroblast cells
GSE66357GSM1683321Illumina NextSeq 500Homo sapiensPS041-2_R2_1_813WI-38 human lung fibroblast cells
GSE66357GSM1683322Illumina NextSeq 500Homo sapiensPS041-2_R2_1_817WI-38 human lung fibroblast cells

Gene rank in WI-38 human lung fibroblast cells

Displaying 131-140 of 18975 results.
Rank orderGene SymbolEnsembl ID
131ITGB1ENSG00000150093
132CAMK2N1ENSG00000162545
133RPL35AENSG00000182899
134UCHL1ENSG00000154277
135CKAP4ENSG00000136026
136YWHABENSG00000166913
137TOMM7ENSG00000196683
138COX7CENSG00000127184
139RPS18ENSG00000096150
140GTF2IENSG00000263001