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  circulating pancreas tumor cell

Overall DesignTo achieve a deep RNA sequencing profile of CTCs at the single cell level, we applied a novel inertial focusing-enhanced device, the CTC-iChip, which allows high efficiency negative depletion of normal blood cells, leaving unattached CTCs in solution where they can be selected and analyzed as single cells (Pubmed ID 23552373). CTCs were then subjected to single cell RNA-sequencing (Pubmed ID 20203668).
SummaryCirculating Tumor Cells (CTCs) are shed from primary tumors into the bloodstream, mediating the hematogenous spread of cancer to distant organs. To determine the relevance of ECM protein expression to human disease, CTCs were isolated from the blood of metastatic PDAC patients and subjected to single cell RNA-sequencing. Analysis of 7 pancreatic CTCs from 3 patients revealed that the majority expressed keratins defining their epithelial origin. A total of 13 of 60 extracellular protein genes enriched in mouse CTCs (see GEO GSE51372) were expressed at high levels (>100 rpm) in at least one human pancreatic CTC. Human SPARC was the only gene found at high levels in all human pancreatic CTCs.
Dataset viewGSE60407

Samples in circulating pancreas tumor cell

Displaying 1-7 of 7 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE60407GSM1478319AB 5500xl Genetic AnalyzerHomo sapiensHP1_2circulating pancreas tumor cell
GSE60407GSM1478320AB 5500xl Genetic AnalyzerHomo sapiensHP1_3circulating pancreas tumor cell
GSE60407GSM1478321AB 5500xl Genetic AnalyzerHomo sapiensHP2_2circulating pancreas tumor cell
GSE60407GSM1478322AB 5500xl Genetic AnalyzerHomo sapiensHP2_3circulating pancreas tumor cell
GSE60407GSM1478323AB 5500xl Genetic AnalyzerHomo sapiensHP2_4circulating pancreas tumor cell
GSE60407GSM1478324AB 5500xl Genetic AnalyzerHomo sapiensHP2_5circulating pancreas tumor cell
GSE60407GSM1478325AB 5500xl Genetic AnalyzerHomo sapiensHP3_1circulating pancreas tumor cell

Gene rank in circulating pancreas tumor cell

Displaying 1-10 of 11561 results.
Rank orderGene SymbolEnsembl ID