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  cultured embryonic stem cells[DCX+][12]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX+][12]

Displaying 1-10 of 263 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2311818Illumina HiSeq 2500Homo sapiens12Dp1a_D08cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311819Illumina HiSeq 2500Homo sapiens12Dp1b_B10cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311820Illumina HiSeq 2500Homo sapiens12Dp1b_D03cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311821Illumina HiSeq 2500Homo sapiens12Dp1b_B05cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311822Illumina HiSeq 2500Homo sapiens12Dp4_B11cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311823Illumina HiSeq 2500Homo sapiens12Dp4_C11cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311824Illumina HiSeq 2500Homo sapiens12Dp4_B10cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311825Illumina HiSeq 2500Homo sapiens12Dp4_C10cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311826Illumina HiSeq 2500Homo sapiens12Dp4_D10cultured embryonic stem cells[DCX+][12]
GSE86977GSM2311827Illumina HiSeq 2500Homo sapiens12Dp4_D09cultured embryonic stem cells[DCX+][12]

Gene rank in cultured embryonic stem cells[DCX+][12]

Displaying 51-60 of 23636 results.
Rank orderGene SymbolEnsembl ID
51FTLENSG00000087086
52RPL35AENSG00000182899
53RPL32ENSG00000144713
54UCHL1ENSG00000154277
55CDKN1CENSG00000129757
56CNTN2ENSG00000184144
57RPS3ENSG00000149273
58RPS29ENSG00000213741
59DPYSL3ENSG00000113657
60EEF2ENSG00000167658