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  cultured embryonic stem cells[DCX+][54]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977

Samples in cultured embryonic stem cells[DCX+][54]

Displaying 11-20 of 495 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2313719Illumina HiSeq 2500Homo sapiens54Dp1b_C11cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313720Illumina HiSeq 2500Homo sapiens54Dp6a_B11cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313721Illumina HiSeq 2500Homo sapiens54Dp6a_E02cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313722Illumina HiSeq 2500Homo sapiens54Dp6b_E06cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313723Illumina HiSeq 2500Homo sapiens54Dp6a_A08cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313724Illumina HiSeq 2500Homo sapiens54Dp6a_A02cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313725Illumina HiSeq 2500Homo sapiens54Dp6a_B04cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313726Illumina HiSeq 2500Homo sapiens54Dp6a_D04cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313727Illumina HiSeq 2500Homo sapiens54Dp6b_E05cultured embryonic stem cells[DCX+][54]
GSE86977GSM2313728Illumina HiSeq 2500Homo sapiens54Dp4b_C06cultured embryonic stem cells[DCX+][54]

Gene rank in cultured embryonic stem cells[DCX+][54]

Displaying 1-10 of 23636 results.
Rank orderGene SymbolEnsembl ID