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  cultured embryonic stem cells[DCX+][54]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX+][54]

Displaying 361-370 of 495 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2314069Illumina HiSeq 2500Homo sapiens54Dp2b_H12cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314070Illumina HiSeq 2500Homo sapiens54Dp2a_B03cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314071Illumina HiSeq 2500Homo sapiens54Dp4a_C09cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314072Illumina HiSeq 2500Homo sapiens54Dp6b_B02cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314073Illumina HiSeq 2500Homo sapiens54Dp6b_B09cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314074Illumina HiSeq 2500Homo sapiens54Dp2a_D06cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314075Illumina HiSeq 2500Homo sapiens54Dp2a_A05cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314076Illumina HiSeq 2500Homo sapiens54Dp2a_C08cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314077Illumina HiSeq 2500Homo sapiens54Dp6a_E09cultured embryonic stem cells[DCX+][54]
GSE86977GSM2314078Illumina HiSeq 2500Homo sapiens54Dp2b_A07cultured embryonic stem cells[DCX+][54]

Gene rank in cultured embryonic stem cells[DCX+][54]

Displaying 1-10 of 23636 results.
Rank orderGene SymbolEnsembl ID
1MALAT1ENSG00000251562
2TUBA1AENSG00000167552
3MAP1BENSG00000131711
4ACTG1ENSG00000184009
5EEF1A1ENSG00000156508
6TMSB10ENSG00000034510
7ACTBENSG00000075624
8STMN2ENSG00000104435
9GNASENSG00000087460
10MIR663BENSG00000221288