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  cultured embryonic stem cells[DCX+][BG121095]

Overall DesignThe transcriptomes of 1846 single cells were profiled by SmartSeq2 at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types. Some cells were positively labeled by a expression of a barcoded viral transgene to help establish clonality (marked by an SK).
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development. During development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development. During development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86982
PMIDNA

Samples in cultured embryonic stem cells[DCX+][BG121095]

Displaying 21-30 of 175 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86982GSM2317076Illumina HiSeq 2500Homo sapiens54Dp2_B10_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317077Illumina HiSeq 2500Homo sapiens54Dp2_B11_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317078Illumina HiSeq 2500Homo sapiens54Dp2_C01_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317079Illumina HiSeq 2500Homo sapiens54Dp2_C02_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317080Illumina HiSeq 2500Homo sapiens54Dp2_C03_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317081Illumina HiSeq 2500Homo sapiens54Dp2_C04_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317082Illumina HiSeq 2500Homo sapiens54Dp2_C05_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317083Illumina HiSeq 2500Homo sapiens54Dp2_C06_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317084Illumina HiSeq 2500Homo sapiens54Dp2_C07_smart-seqcultured embryonic stem cells[DCX+][BG121095]
GSE86982GSM2317085Illumina HiSeq 2500Homo sapiens54Dp2_C08_smart-seqcultured embryonic stem cells[DCX+][BG121095]

Gene rank in cultured embryonic stem cells[DCX+][BG121095]

Displaying 31-40 of 23045 results.
Rank orderGene SymbolEnsembl ID
31RPS3AENSG00000145425
32RPL7ENSG00000147604
33MIFENSG00000240972
34C5orf13
35FTH1ENSG00000167996
36CD24ENSG00000272398
37H3F3AENSG00000163041
38RPS13ENSG00000110700
39RPS25ENSG00000118181
40MLLT11ENSG00000213190