Loading

Cell View

Cell Information

  cultured embryonic stem cells[DCX-][12]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][12]

Displaying 241-241 of 241 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2312125Illumina HiSeq 2500Homo sapiens12Dn4_H11cultured embryonic stem cells[DCX-][12]

Gene rank in cultured embryonic stem cells[DCX-][12]

Displaying 23601-23610 of 23636 results.
Rank orderGene SymbolEnsembl ID
23601WDR88ENSG00000166359
23602WEE2ENSG00000214102
23603WFDC10AENSG00000180305
23604WFDC11ENSG00000180083
23605WFDC5ENSG00000175121
23606WFDC8ENSG00000158901
23607WNT9AENSG00000143816
23608WNT9BENSG00000158955
23609XAGE1AENSG00000204379
23610XAGE1BENSG00000204382