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  cultured embryonic stem cells[DCX-][12]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][12]

Displaying 221-230 of 241 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2312133Illumina HiSeq 2500Homo sapiens12Dn4_B07cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312134Illumina HiSeq 2500Homo sapiens12Dn4_A06cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312135Illumina HiSeq 2500Homo sapiens12Dn4_B06cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312136Illumina HiSeq 2500Homo sapiens12Dn4_B05cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312137Illumina HiSeq 2500Homo sapiens12Dn4_A05cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312138Illumina HiSeq 2500Homo sapiens12Dn4_F04cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312139Illumina HiSeq 2500Homo sapiens12Dn4_D05cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312140Illumina HiSeq 2500Homo sapiens12Dn4_B04cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312141Illumina HiSeq 2500Homo sapiens12Dn4_G02cultured embryonic stem cells[DCX-][12]
GSE86977GSM2312142Illumina HiSeq 2500Homo sapiens12Dn4_A03cultured embryonic stem cells[DCX-][12]

Gene rank in cultured embryonic stem cells[DCX-][12]

Displaying 23541-23550 of 23636 results.
Rank orderGene SymbolEnsembl ID
23541TTTY6BENSG00000131548
23542TTTY7BENSG00000147761
23543TTTY8ENSG00000183385
23544TTTY9A
23545TYRENSG00000077498
23546TYROBPENSG00000011600
23547TYRP1ENSG00000107165
23548UAP1L1ENSG00000197355
23549UBASH3AENSG00000160185
23550UBL4BENSG00000186150