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  cultured embryonic stem cells[DCX-][12]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][12]

Displaying 241-241 of 241 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2312153Illumina HiSeq 2500Homo sapiens12Dn3_F11cultured embryonic stem cells[DCX-][12]

Gene rank in cultured embryonic stem cells[DCX-][12]

Displaying 23551-23560 of 23636 results.
Rank orderGene SymbolEnsembl ID
23551UBQLN3ENSG00000175520
23552UCN2ENSG00000145040
23553UCP1ENSG00000109424
23554UGT1A1ENSG00000241635
23555UGT1A10ENSG00000242515
23556UGT1A3ENSG00000243135
23557UGT1A4ENSG00000244474
23558UGT1A5ENSG00000240224
23559UGT1A7ENSG00000244122
23560UGT2B11ENSG00000213759