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  cultured embryonic stem cells[DCX-][26]

Overall DesignThe transcriptomes of 2684 single cells were profiled by CelSeq at different timepoints throughout a 54-day differentiation protocol that converted H1 human embryonic stem cells to a variety of brain cell types.
SummaryDuring development of the human brain, multiple cell types with diverse regional identities are generated. Here we report a system to generate early human brain forebrain and mid/hindbrain cell types from human embryonic stem cells (hESCs), and infer and experimentally confirm a lineage tree for the generation of these types based on single-cell RNA-Seq analysis. We engineered SOX2Cit/+ and DCXCit/Y hESC lines to target progenitors and neurons throughout neural differentiation for single-cell transcriptomic profiling, then identified discrete cell types consisting of both rostral (cortical) and caudal (mid/hindbrain) identities. Direct comparison of the cell types were made to primary tissues using gene expression atlases and fetal human brain single-cell gene expression data, and this established that the cell types resembled early human brain cell types, including preplate cells. From the single-cell transcriptomic data a Bayesian algorithm generated a unified lineage tree, and predicted novel regulatory transcription factors. The lineage tree highlighted a prominent bifurcation between cortical and mid/hindbrain cell types, confirmed by clonal analysis experiments. We demonstrated that cell types from either branch could preferentially generated by manipulation of the canonical Wnt/beta-catenin pathway. In summary, we present an experimentally validated lineage tree that encompasses multiple brain regions, and our work sheds light on the molecular regulation of region-specific neural lineages during human brain development.
Dataset viewGSE86977
PMIDNA

Samples in cultured embryonic stem cells[DCX-][26]

Displaying 41-50 of 85 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE86977GSM2313076Illumina HiSeq 2500Homo sapiens26Dn1a_D11cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313075Illumina HiSeq 2500Homo sapiens26Dn2_A01cultured embryonic stem cells[DCX-][26]
GSE86977GSM2312819Illumina HiSeq 2500Homo sapiens26Dn2_A02cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313175Illumina HiSeq 2500Homo sapiens26Dn2_A03cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313068Illumina HiSeq 2500Homo sapiens26Dn2_A04cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313066Illumina HiSeq 2500Homo sapiens26Dn2_A05cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313061Illumina HiSeq 2500Homo sapiens26Dn2_A06cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313180Illumina HiSeq 2500Homo sapiens26Dn2_A07cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313186Illumina HiSeq 2500Homo sapiens26Dn2_A08cultured embryonic stem cells[DCX-][26]
GSE86977GSM2313056Illumina HiSeq 2500Homo sapiens26Dn2_A09cultured embryonic stem cells[DCX-][26]

Gene rank in cultured embryonic stem cells[DCX-][26]

Displaying 23611-23620 of 23636 results.
Rank orderGene SymbolEnsembl ID
23611ZAP70ENSG00000115085
23612ZBED2ENSG00000177494
23613ZC2HC1BENSG00000118491
23614ZC3HAV1LENSG00000146858
23615ZFAT-AS1ENSG00000248492
23616ZFP91-CNTFENSG00000255073
23617ZFP92ENSG00000189420
23618ZNF20ENSG00000132010
23619ZNF239ENSG00000196793
23620ZNF295-AS1ENSG00000237232