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  cultured embryonic stem cells (H1) DAPI- Live cells FRSCR

Overall Design26 Llive and 19 Fixed cultured hESCs were prepared and sequenced using both FRISCR and TritonX-100 Lysis as proof of principal for FRSCR.
SummaryThe human neocortex is created from diverse progenitors that are intermixed with multiple cell types in the prenatal germinal zones. These progenitors have been difficult to profile with unbiased transcriptomics since progenitors-particularly radial glia (RG)-are rare cell types, defined by a combination of intracellular markers, position and morphology. To circumvent these problems, we developed a method called FRSCR for transcriptome profiling of individual fixed, stained, and sorted cells. After validation of FRSCR with human embryonic stem cells, we profiled primary human RG that constitute only 1% of the mid-gestation cortex. These data showed that RG could be classified into ventricle zone-enriched RG (vRG) that expressed ANXA1 and CRYAB, and outer subventricular zone-localized RG (oRG) that expressed HOPX. Our study identified the first markers and molecular profiles of vRG and oRG cells, and provides an essential step for understanding molecular networks that control the development and lineage of human neocortical progenitors. Furthermore, FRSCR allows targeted single-cell transcriptomic profiling of many tissues that currently lack live-cell markers.
Dataset viewGSE71858
PMID26524239

Samples in cultured embryonic stem cells (H1) DAPI- Live cells FRSCR

Displaying 1-10 of 15 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE71858GSM1846949Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-10]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846950Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-11]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846951Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-12]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846952Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-13]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846953Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-14]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846954Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-15]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846955Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-16]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846956Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457350-17]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846972Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457351-10]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR
GSE71858GSM1846973Illumina HiSeq 2500Homo sapiensLive_PLRC [US-1457351-11]cultured embryonic stem cells (H1) DAPI- Live cells FRSCR

Gene rank in cultured embryonic stem cells (H1) DAPI- Live cells FRSCR

Displaying 21-30 of 17315 results.
Rank orderGene SymbolEnsembl ID
21PFN1ENSG00000108518
22MIFENSG00000240972
23RPL3ENSG00000100316
24H2AFZENSG00000164032
25RPL31ENSG00000071082
26RPS6ENSG00000137154
27RANENSG00000132341
28RPS3AENSG00000145425
29RPS3ENSG00000149273
30NPM1ENSG00000181163