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  d2 AG+ cells

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in d2 AG+ cells

Displaying 1-2 of 2 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643170AB 5500xl Genetic AnalyzerHomo sapiensd2_wt1d2 AG+ cells
GSE67259GSM1643171AB 5500xl Genetic AnalyzerHomo sapiensd2_wt2d2 AG+ cells

Gene rank in d2 AG+ cells

Displaying 11-20 of 20748 results.
Rank orderGene SymbolEnsembl ID
11RPS24ENSG00000138326
12RPL27AENSG00000166441
13FTH1ENSG00000167996
14HSP90AA1ENSG00000080824
15RPS20ENSG00000008988
16CTSL2
17PPIAENSG00000196262
18RPL3ENSG00000100316
19RPL13AENSG00000142541
20TUBBENSG00000183311