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  d6 CSM+ cells

Overall DesignRNAseq analysis of human induced pluripotent stem cells (hiPSC), incipient mesoderm-like cells (iMeLC) and primordial germ cell-like cells (BLIMP1-2A-tdTomato (BT)/TFAP2C-2A-ECFP (AG) expressing cells or EpCAM/CD49f (CSM) positive cells)
SummaryMechanisms underlying human germ cell development are unclear, partly due to difficulties in studying human embryos and lack of suitable experimental systems. Here, we show that human induced pluripotent stem cells (hiPSCs) differentiate into incipient mesoderm-like cells (iMeLCs), which robustly generate human primordial germ cell-like cells (hPGCLCs) that can be purified using the surface markers EpCAM and INTEGRINα6. The transcriptomes of hPGCLCs and primordial germ cells (PGCs) isolated from non-human primates are similar, and although specification of hPGCLCs and mouse PGCs rely on similar signaling pathways, hPGCLC specification transcriptionally activates germline fate without transiently inducing eminent somatic programs. This includes genes important for naive pluripotency and repression of key epigenetic modifiers, concomitant with epigenetic reprogramming. Accordingly, BLIMP1, which represses somatic programs in mice, activates and stabilizes a germline transcriptional circuit and represses a default neuronal differentiation program. Together, these findings provide a foundation for understanding and reconstituting human germ cell development in vitro.
Dataset viewGSE67259
PMID26189426

Samples in d6 CSM+ cells

Displaying 1-2 of 2 results.
SeriesSampleInstrumentOrganismTitleCell Source
GSE67259GSM1643163AB 5500xl Genetic AnalyzerHomo sapiens585A1_surface_d6#1d6 CSM+ cells
GSE67259GSM1643164AB 5500xl Genetic AnalyzerHomo sapiens585A1_surface_d6#2d6 CSM+ cells

Gene rank in d6 CSM+ cells

Displaying 51-60 of 20748 results.
Rank orderGene SymbolEnsembl ID
51RPLP2ENSG00000177600
52TUBBENSG00000183311
53RPL21ENSG00000122026
54RPL10ENSG00000147403
55RPL4ENSG00000174444
56RPL12ENSG00000197958
57RPS13ENSG00000110700
58RPS16ENSG00000105193
59RPSAENSG00000168028
60RPS25ENSG00000118181